Search of all papers from the Leach lab
2021
Gonzalez-Fernandez T, Tenorio AJ, Saiz AM, Leach JK. Engineered Cell-Secreted Extracellular Matrix Modulates Cell Spheroid Mechanosensing and Amplifies their Response to Inductive Cues for the Formation of Mineralized Tissues. Advanced Healthcare Materials. 2021 December 30. doi.org/10.1002/adhm.202102337
Gionet-Gonzales M, Casella A, Diloretto D, Ginnell C, Griffin KH, Bigot A, Leach JK. Sulfated Alginate Hydrogels Prolong the Therapeutic Potential of MSC Spheroids by Sequestering the Secretome. Advanced Healthcare Materials. 2021 September 05. doi: https://doi.org/10.1002/adhm.202101048
2020
Gresham RCH, Bahney CS, Leach JK. Growth factor delivery using extracellular matrix-mimicking substrates for musculoskeletal tissue engineering and repair. Bioactive Materials. 2020 December 16. doi: https://doi.org/10.1016/j.bioactmat.2020.12.012
Whitehead J, Griffin KH, Gionet-Gonzales M, Vorwald CE, Cinque SE, Leach JK. Hydrogel mechanics are a key driver of bone formation by mesenchymal stromal cell spheroids. Biomaterials. 2020 December 14. doi: https://doi.org/10.1016/j.biomaterials.2020.120607
Thai VL, Griffin KH, Thorpe SW, Randall RL, Leach JK. Tissue engineered platforms for studying primary and metastatic neoplasm behavior in bone. Journal of Biomechanics. 2020 December 11. doi: https://doi.org/10.1016/j.jbiomech.2020.110189
Gonzalez-Fernandez T, Tenorio AJ, Campbell K, Silva E, Leach JK. Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts. Tissue Engineering Part A. 2020 November 20. doi: 10.1089/ten.TEA.2020.0305
Casella A, Panitch A, Leach JK. Endogenous electric signaling as a blueprint for conductive materials in tissue engineering. Bioelectricity. 2020 Oct 15. doi: 10.1089/bioe.2020.0027
Gonzalez-Fernandez T, Tenorio AJ, Leach JK. Three-dimensional printed stamps for the fabrication of patterned microwells and high-throughput production of homogeneous cell spheroids. 3D Printing and Additive Manufacturing. 2020 May 06. doi: 10.1089/3dp.2019.0129
Hung BP, Gonzalez-Fernandez T, Lin JB, Campbell T, Lee YB, Panitch A, Alsberg E, Leach JK. Multi-peptide presentation and hydrogel mechanics jointly enhance therapeutic duo-potential of entrapped stromal cells. Biomaterials. 2020 Mar 13. doi.org/10.1016/j.biomaterials.2020.119973.
Vorwald CE, Gonzalez-Fernandez T, Joshee S, Sikorski P, Leach JK. Tunable fibrin-alginate interpenetrating network hydrogels to support cell spreading and network formation. Acta Biomater. 2020 Mar 10. doi: 10.1016/j.actbio.2020.03.014.
Lowen JM, Leach JK. Functionally graded biomaterials for use as model systems and replacement tissues. Adv. Funct. Mater. 2020 Mar 04. doi: 10.1002/adfm.201909089.
Harvestine JN, Gonzalez-Fernandez T, Sebastian A, Hum NR, Genetos DC, Loots, GG, Leach JK. Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates. Science Advances. 2020 Feb 12. doi: 10.1126/sciadv.aay2387.
Vorwald CE, Joshee S, Leach JK. Spatial localization of endothelial cells in heterotypic spheroids influences Notch signaling. J Mol Med. 2020 Jan 23. doi: 0.1007/s00109-020-01883-1.
2019
Whitehead J, Kothambawala A, Leach JK. Morphogen delivery by osteoconductive nanoparticles instructs stromal cell spheroid phenotype. Adv. Biosys. 2019 Oct 01. doi: 10.1002/adbi.201900141.
Whitehead J, Zhang J, Harvestine JN, Kothambawala A, Liu GY, Leach JK. Tunneling nanotubes mediate the expression of senescence markers in mesenchymal stem/stromal cell spheroids. Stem Cells. 2019 Jul 12. doi: 10.1002/stem.3056.
Gonzalez-Fernandez T, Sikorski P, Leach JK. Bio-instructive materials for musculoskeletal regeneration. Acta Biomater. 2019 Jul 11. pii: S1742-7061(19)30494-5. doi: 10.1016/j.actbio.2019.07.014.
Saiz AM Jr, Gionet-Gonzales MA, Lee MA, Leach JK. Conditioning of myoblast secretome using mesenchymal stem/stromal cell spheroids improves bone repair. Bone. 2019 Aug;125:151-159. doi: 10.1016/j.bone.2019.05.018. Epub 2019 May 16.
Harvestine JN, Saiz AM, Leach JK. Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts. Biomater Sci. 2019 Apr 23;7(5):2091-2101. doi: 10.1039/c8bm01478g.
Ingavle GC, Gionet-Gonzales M, Vorwald CE, Bohannon LK, Clark K, Galuppo LD, Leach JK. Injectable mineralized microsphere-loaded composite hydrogels for bone repair in a sheep bone defect model. Biomaterials. 2019 Mar;197:119-128. doi: 10.1016/j.biomaterials.2019.01.005. Epub 2019 Jan 10.
2018
Hung BP, Harvestine JN, Saiz AM, Gonzalez-Fernandez T, Sahar DE, Weiss ML, Leach JK. Defining hydrogel properties to instruct lineage-and cell-specific mesenchymal differentiation. Biomaterials. Oct 2018; 189:1-10. doi: 10.1016/j.biomaterials.2018.10.024. Epub 2018 Oct 22.
Zhang J, Whitehead J, Liu Y, Yang Q, Leach JK, Liu GY. Direct observation of tunneling nanotubes within human mesenchymal stem cell spheroids. J Phys Chem B. 2018 Oct 23. doi: 10.1021/acs.jpcb.8b07305.
Harvestine JN, Orbay H, Chen JY, Sahar DE, Leach JK. Cell-secreted extracellular matrix, independent of cell source, promotes the osteogenic differentiation of human stromal vascular fraction. J. Mater. Chem. B. 2018,6, 4104-4115. doi: 10.1039/C7TB02787G.
Vorwald CE, Murphy KC, Leach JK. Restoring vasculogenic potential of endothelial cells from diabetic patients through spheroid formation.Cell Mol Bioeng. 2018 Aug;11(4):267-278. doi: 10.1007/s12195-018-0531-1. Epub 2018 May 23.
Mitra D, Yasui OW, Harvestine JN, Link JM, Hu JC, Athanasiou KA, Leach JK. Exogenous lysyl oxidase-like 2 and perfusion culture induce collagen crosslink formation in osteogenic grafts. Biotechnol J. 2018 Jul 27:e1700763. doi: 10.1002/biot.201700763.
Ho SS, Hung BP, Heyrani N, Lee MA, Leach JK. Hypoxic Preconditioning of mesenchymal stem cells with subsequent spheroid formation accelerates repair of segmental bone defects. Stem Cells. 2018 Sep;36(9):1393-1403. doi: 10.1002/stem.2853. Epub 2018 Jul 3.
Vorwald CE, Ho SS, Whitehead J, Leach JK. High-throughput formation of mesenchymal stem cell spheroids and entrapment in alginate hydrogels. Methods Mol Biol. 2018;1758:139-149. doi: 10.1007/978-1-4939-7741-3_11.
Sherlock BE, Harvestine JN, Mitra D, Haudenschild A, Hu J, Athanasiou KA, Leach JK, Marcu L. Nondestructive assessment of collagen hydrogel cross-linking using time-resolved autofluorescence imaging.J Biomed Opt. 2018 Mar;23(3):1-9. doi: 10.1117/1.JBO.23.3.036004.
Gionet-Gonzales M and Leach JK. Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin. Biomed Mater. 2018 Mar 21;13(3):034109. doi: 10.1088/1748-605X/aab0b3.
2017
Ho SS, Keown AT, Addison B, Leach JK. Cell migration and bone formation from mesenchymal stem cell spheroids in alginate hydrogels are regulated by adhesive ligand density. Biomacromolecules. 2017 Dec 11;18(12):4331-4340. doi: 10.1021/acs.biomac.7b01366. Epub 2017 Nov 30.
Murphy KC, Whitehead J, Zhou D, Ho SS, Leach JK. Engineering fibrin hydrogels to promote the wound healing potential of mesenchymal stem cell spheroids. Acta Biomater. 2017 Dec;64:176-186. doi: 10.1016/j.actbio.2017.10.007. Epub 2017 Oct 5.
Mitra D, Fatakdawala H, Nguyen-Truong M, Creecy A, Nyman J, Marcu L, Leach JK. Detection of pentosidine cross-links in cell-secreted decellularized matrices using time resolved fluorescence spectroscopy. ACS Biomater Sci Eng. 2017 Sep 11;3(9):1944-1954. doi: 10.1021/acsbiomaterials.6b00029. Epub 2016 May 31.
Mitra D, Whitehead J, Yasui OW, Leach JK. Bioreactor culture duration of engineered constructs influences bone formation by mesenchymal stem cells. Biomaterials. 2017 Nov;146:29-39. doi: 10.1016/j.biomaterials.2017.08.044. Epub 2017 Sep 6.
Leach JK and Whitehead J. Materials-directed differentiation of mesenchymal stem cells for tissue engineering and regeneration. ACS Biomater. Sci. Eng. 2017 Mar 14, Article ASAP, doi: 10.1021/acsbiomaterials.6b00741.
Murphy KC, Whitehead J, Falahee PC, Zhou D, Simon SI, Leach JK. Multifactorial experimental design to optimize the anti-inflammatory and proangiogenic potential of mesenchymal stem cell spheroids. Stem Cells. 2017 Jun;35(6):1493-1504. doi: 10.1002/stem.2606. Epub 2017 Mar 27.
Murphy KC, Hung BP, Browne-Bourne S, Zhou D, Yeung J, Genetos DC, Leach JK. Measurement of oxygen tension within mesenchymal stem cell spheroids. Journal of the Royal Society Interface. 2017 Feb;14(127). pii: 20160851. doi: 10.1098/rsif.2016.0851.
2016
Harvestine JN, Vollmer NL, Ho SS, Zikry CA, Lee MA, Leach JK. Extracellular matrix-coated composite scaffolds promote mesenchymal stem cell persistence and osteogenesis. Biomacromolecules. 2016 Nov 14;17(11):3524-3531.
Ho SS, Vollmer NL, Refaat MI, Jeon O, Alsberg E, Lee MA, Leach JK. Bone morphogenetic protein-2 promotes human mesenchymal stem cell survival and resultant bone formation when entrapped in photocrosslinked alginate hydrogels. Adv Healthc Mater. 2016 Sep 1.
Murphy KC, Hoch AI, Harvestine JN, Zhou D, Leach JK. Mesenchymal stem cell spheroids retain osteogenic phenotype through α2β1 signaling. Stem Cells Transl Med. 2016 Jun 30. pii: sctm.2015-0412.
Ho SS, Murphy KC, Binder BY, Vissers CB, Leach JK. Increased survival and function of mesenchymal stem cell spheroids entrapped in instructive alginate hydrogels. Stem Cells Transl Med. 2016 Jun;5(6):773-81.
Hoch AI, Mittal V, Mitra D, Vollmer N, Zikry CA, Leach JK. Cell-secreted matrices perpetuate the bone-forming phenotype of differentiated mesenchymal stem cells. Biomaterials. 2016 Jan 9;74:178-187.
2015
Vissers CAB, Harvestine JN, Leach JK. Pore size regulates mesenchymal stem cell response to Bioglass-loaded composite scaffolds. J. Mater. Chem. B. 2015 Sept 29.
Murphy KC, Stilhano RS, Mitra D, Zhou D, Batarni S, Silva EA, Leach JK. Hydrogel biophysical properties instruct coculture-mediated osteogenic potential. FASEB J. 2015 Oct 6. pii: fj.15-279984.
Binder B, Williams P, Silva E, Leach JK. Lysophosphatidic acid and sphingosine-1-phosphate: a concise review of biological function and applications for tissue engineering. Tissue Eng Part B Rev. 2015 Jun 2.
2014
Murphy KC, Hughbanks ML, Binder BY, Vissers CB, Leach JK. Engineered fibrin gels for parallel stimulation of mesenchymal stem cell proangiogenic and osteogenic potential. Ann Biomed Eng. 2015 Aug;43(8):2010-21.
Binder BY, Leach JK, Marcu L. Fluorescence tools for use in tissue engineering. in Fluorescent Lifetime Spectroscopy of Imaging in Tissue Biomedical Diagnostics. Marcu L, French PMW, and Elson DW, editors. 2014, CRC Press.
Binder BY, Sagun JE, Leach JK. Reduced serum and hypoxic culture conditions enhance the osteogenic potential of human mesenchymal stem cells. Stem Cell Rev. 2015 Jun;11(3):387-93.
Murphy KC, Fang SY, Leach JK. Human mesenchymal stem cell spheroids in fibrin hydrogels exhibit improved cell survival and potential for bone healing. Cell Tissue Res. 2014 Jul;357(1):91-9.
Hoch AI, Leach JK. Concise Review: Optimizing expansion of none marrow mesenchymal stem/stromal cells for clinical applications. Stem Cells Transl Med. 2014 May;3(5):643-52.
Textor JA, Murphy KC, Leach JK, Tablin F. Ultrastructure and growth factor content of equine platelet-rich fibrin gels. Am J Vet Res. 2014 Apr;75(4):392-401.
Soicher MA, Christiansen BA, Stover SM, Leach JK, Yellowley CE, Griffiths LG, Fyhrie DP. Remineralized bone matrix as a scaffold for bone tissue engineering. J Biomed Mater Res A. 2014 Dec;102(12):4480-90.
Man AJ, Leach JK, Bannerman P. Redirection of neurite outgrowth by coupling chondroitin sulfate proteoglycans to polymer membranes. Ann Biomed Eng. 2014 Jun;42(6):1271-81.
Jose S, Hughbanks ML, Binder BY, Ingavle GC, Leach JK. Enhanced trophic factor secretion by mesenchymal stem/stromal cells with glycine-histidine-lysine (GHK)-modified alginate hydrogels. Acta Biomater. 2014 May;10(5):1955-64.
2013
p>Binder BY, Sondergaard CS, Nolta JA, Leach JK. Lysophosphatidic Acid enhances stromal cell-directed angiogenesis. PLoS One. 2013 Dec 2;8(12):e82134.
Binder BY, Genetos DC, Leach JK. Lysophosphatidic acid protects human mesenchymal stromal cells from differentiation-dependent vulnerability to apoptosis. Tissue Eng Part A. 2014 Apr;20(7-8):1156-64.
Bhat A, Hoch AI, Decaris ML, Leach JK. Alginate hydrogels containing cell-interactive beads for bone formation. FASEB J. 2013 Dec;27(12):4844-52.
Ingavle G, Leach JK. Advancements in electrospinning of polymeric nanofibrous scaffolds for tissue engineering. Tissue Eng Part B Rev. 2014 Aug;20(4):277-93.
Davis HE, Binder BY, Schaecher P, Yakoobinsky DD, Bhat A, Leach JK. Enhancing osteoconductivity of fibrin gels with apatite-coated polymer microspheres. Tissue Engineering Part A. 2013; 19:1773–1782.
2012
Murphy KC, Leach JK. A reproducible, high throughput method for fabricating fibrin gels. BMC Res Notes. 2012 August;5:423.
Decaris M, Binder BY, Soicher M, Bhat A, Leach JK. Cell-derived matrix coatings for polymeric scaffolds. Tissue Engineering Part A. 2012 Oct;18(19-20):2148-57.
Morales-Hernandez DG, Genetos DC, Murphy KC, Leach JK. Ceramic identity contributes to mechanical properties and osteoblast behavior on macroporous composite scaffolds. J. Funct. Biomater. 2012 May;3(2), 382-397.
He J, Decaris M, Leach JK. Bioceramic-mediated trophic factor secretion by mesenchymal stem cells enhances in vitro endothelial cell persistence and in vivo angiogenesis. Tissue Engineering Part A. 2012 Jul;18(13-14):1520-8.
Hoch AI, Binder BY, Genetos DC, Leach JK. Differentiation-dependent secretion of proangiogenic factors by mesenchymal stem cells. PLoS One. 2012 April;7(4):e35579.
Decaris ML, Mojadedi A, Bhat A, Leach JK. Transferable cell-secreted extracellular matrices enhance osteogenic differentiation. Acta Biomaterialia. 2012 Feb;8(2):744-52.
2011
Bhat A, Boyadjiev SA, Senders CW, Leach JK. Differential growth factor adsorption to calvarial osteoblast-secreted extracellular matrices instructs osteoblastic behavior. PLoS One. 2011;6(10):e25990.
Man A, Davis HE, Ito A, Leach JK, Bannerman P. Neurite outgrowth in fibrin gels is regulated by substrate stiffness. Tissue Eng Part A. 2011 Dec;17(23-24):2931-42.
Davis HE, Case EM, Miller SL, Genetos DC, Leach JK. Osteogenic response to BMP-2 of hMSCs grown on apatite-coated scaffolds. Biotechnol Bioeng. 2011 Nov;108(11):2727-35.
Fite BZ, Decaris M, Sun Y, Sun Y, Lam A, Ho CKL, Leach JK, Marcu L. Noninvasive multimodal evaluation of bioengineered cartilage constructs combining time-resolved fluorescence and ultrasound imaging. Tissue Eng Part C Methods. 2011 Apr;17(4):495-504.
Decaris ML, Leach JK. Design of experiments approach to engineer cell-secreted matrices for directing osteogenic differentiation. Ann Biomed Eng. 2011 Apr;39(4):1174-85.
Davis HE, Miller SL, Case EM, Leach JK. Supplementation of fibrin gels with sodium chloride enhances physical properties and ensuing osteogenic response. Acta Biomater. 2011 Feb;7(2):691-9.
Davis HE, Leach JK. Designing bioactive delivery systems for tissue regeneration. Ann Biomed Eng. 2011 Jan;39(1):1-13.
2010
Cheung WK, Working DM, Galuppo LD, Leach JK. Osteogenic comparison of expanded and uncultured adipose stromal cells. Cytotherapy. 2010 Jul; 12(4):554-62.
He J, Genetos DC, Yellowley CE, Leach JK. Oxygen tension differentially influences osteogenic differentiation of human adipose stem cells in 2D and 3D cultures. J Cell Biochem. 2010 May;110(1):87-96.
Rao RR, He J, Leach JK. Biomineralized composite substrates increase gene expression with nonviral delivery. J Biomed Mater Res Part A. 2010 Aug;94(2):344-54.
Genetos DC, Cheung WK, Decaris ML, Leach JK. Oxygen tension modulates neurite outgrowth in PC12 cells through a mechanism involving HIF and VEGF. J Mol Neurosci. 2010 Mar;40(3):360-6.
He J, Genetos DC, Leach JK. Osteogenesis and trophic factor secretion are influenced by the composition of hydroxyapatite/ poly(lactide-co-glycolide) composite scaffolds. Tissue Eng Part A. 2010 Jan;16(1):127-37.
2009
Decaris ML, Lee CI, Yoder MC, Tarantal AF, Leach JK. Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells. Angiogenesis 2009;12(4):303-11.
Leu A, Steiger SM, Dayton PA, Ferrara KW, Leach JK. Angiogenic response to bioactive glass promotes bone healing in an irradiated calvarial defect. Tissue Eng 2009;15(4): 877-885.
Davis HE, Rao RR, He, J, Leach JK. Biomimetic scaffolds fabricated from apatite-coated microspheres. J Biomed Mater Res Part A; 2009 Sep 15;90(4):1021-31.
2008
Leu A, Leach JK. Proangiogenic potential of a collagen/bioactive glass substrate. Pharm Res 2008;25(5):1222-1229.
Leach JK and Mooney DJ. Synthetic extracellular matrices (editorial). Pharm Res 2008;25(5):1209-1211.
Davis HE and Leach JK. Hybrid and composite biomaterials in tissue engineering. Topics in Multifunctional Biomaterials and Devices e-book, Vol. 1, N. Ashammakhi, Ed, 2008.
Leach JK. Keeping an eye on the prize: The need for noninvasive imaging in tissue engineering. Tissue Engineering Research, Nova Science Publishers, Hauppauge, NY, G. Greco, Ed, 2008, pp. 1-6.
Rocha FG, Sundback CA, Krebs NJ, Leach JK, Mooney DJ, Ashley SW, Vacanti JP, Whang EE. The effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine. Biomaterials 2008;29(19):2884-90.
2007
Riddle KW, Kong HJ, Leach JK, Fischbach C, Cheung C, Anseth KS, Mooney DJ. Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo. Mol Ther 2007:15(2):361-368.
2006
Leach JK. Multifunctional cell-instructive materials for tissue regeneration. Regen Med 2006;1(4):447-455.
Leach JK, Kaigler D, Wang Z, Krebsbach PH, Mooney DJ. Coating of VEGF-releasing scaffolds with bioactive glass for angiogenesis and bone regeneration. Biomaterials 2006:27:3249-3255.
Sedrakyan S, Zhou ZY, Perin L, Leach K, Mooney D, Kim TH. Tissue engineering of a small hand phalanx with a porously casted polylactic acid-polyglycolic acid copolymer. Tissue Eng 2006;12(9):2675-83.